Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV022225 GenBank Accession EU189112
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 615
Definition Human bocavirus isolate ZA29-04 capsid protein (VP1/2) gene, partial cds. Submission Date 2007-10-02
Strain Name ZA29-04 Sampling Date from 2004-05 to 2005-11
Sampling Country South Africa Location Red Cross Children’s Hospital, South Africa, South Africa
Submitted By Smuts,H.E., Workman,L. and Zar,H.J. Submitting Institution Div. Medical Virology/NHLS, Dept. Clinical Laboratory Sciences, University of Cape Town, Anzio Road, Observatory, Cape Town, Western Cape 7925, South Africa
Original Host Homo sapiens Sample Type nasal swab
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Nasal swab Collection of respiratory samples from children. 18360904 View Related Publication
2 Viral transport medium Preservation and transport of nasopharyngeal swab samples to the laboratory.
3 Monoclonal antibodies Detection of RSV, influenza A and B viruses, adenovirus, and parainfluenza viruses 1, 2, and 3 in shell vial culture.
4 Light Diagnostics indirect immunofluorescence assay Identification of specific viruses in the shell vial culture samples.
5 Talent Seek Viral RNA kit (Talent Sri, Trieste, Italy) Extraction of RNA from respiratory samples.
6 Random primers (Roche Diagnostics, Penzberg, Germany) Conversion of purified RNA into cDNA.
7 iScript cDNA synthesis kit (Bio-Rad, Hercules, CA) Conversion of purified RNA into cDNA.
8 QIAquick PCR purification kit (Qiagen, Hilden, Germany) Purification of PCR products for sequencing.
9 ABI 310 sequencer with a fluorescent dye terminator kit (Applied Biosystem, Foster City, CA) Sequencing of purified PCR products.