Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV022203 GenBank Accession DQ317545
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 680
Definition Human bocavirus isolate 125/04 capsid protein (VP1/2) gene, partial cds. Submission Date 2005-12-07
Strain Name 125/04 Sampling Date 2004
Sampling Country South Africa Location CapeTown, South Africa
Submitted By Smuts,H.E. Submitting Institution Medical Virology, University of Cape Town, Anzio Road, Observatory, Cape Town, Western Cape 7925, South Africa
Original Host None Sample Type nasopharyngeal and bronchoalveolar lavage samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Indirect Immunofluorescence Assay (Light Diagnostics, Chemicon International, Temecula, CA, USA) Screening for common respiratory viruses including respiratory syncytial virus, influenza virus A and B, parainfluenza viruses 1, 2, and 3, adenovirus, and cytomegalovirus. 17073104 View Related Publication
2 Reverse Transcription-PCR Detection of HMPV and HCoV NL63.
3 QIAamp DNA Blood Mini Kit (Qiagen Inc., Valencia, CA, USA) Extraction of DNA.
4 Supertherm Polymerase (JMR Holdings, Kent, UK) PCR amplification.
5 MgCl2 PCR amplification component.
6 dNTPs PCR amplification component.
7 Primers NP-1 s1 (5'-TAACTGCTCCAGCAAGTCCTCCA) and NP-1 as1 (5'-GGAAGCTCTGTGTTGACTGAAT) Primary PCR amplification of the NP-1 gene.
8 Primers NP-1 as1 and NP-1 s2 (5'-CTCACCTGCGAGCTCTGTAAGTA) Secondary semi-nested PCR to improve sensitivity.
9 Primers VP s1 (5'-GCACTTCTGTATCAGATGCCTT), VP as1 (5'-CGTGGTATGTAGGCGTGTAG), and VP s2 (5'-CTTAGAACTGGTGAGAGCACTG) PCR amplification of the VP1/2 capsid gene for confirming HBoV.