Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV022150 | GenBank Accession | KJ206565 |
| Subfamily | Parvovirinae | Genus | unclassified Parvovirinae |
| Species | Bufavirus VS6600009 | Sequence Length | 336 |
| Definition | Bufavirus VS6600009 VP2 gene, partial cds. | Submission Date | 2014-01-28 |
| Strain Name | VS6600009 | Sampling Date | from 2005 to 2009 |
| Sampling Country | Netherlands | Location | the reference laboratory for viral gastroenteritis at the National Institute for Public Health and the Environment, Bilthoven, the Netherlands, Netherlands |
| Submitted By | Smits,S.L., Schapendonk,C.M.E., Schipper,D., Bodewes,R., Haagmans,B.L., Osterhaus,A.D.M.E. and Koopmans,M. | Submitting Institution | Viroscience, Erasmus Medical Center, Dr Molewaterplein 50, Rotterdam 3015 GE, The Netherlands |
| Original Host | Homo sapiens | Sample Type | stool samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Stool samples | Obtained from patients who had acute gastroenteritis of unknown etiology; analyzed for (un)known viruses using a metagenomics approach. | 24964003 | View Related Publication |
| 2 | BLAST searches | Used to characterize 169,305 trimmed reads for the presence of viral sequences. | ||
| 3 | 454-sequencing (Roche, Basel, Switzerland) | Used to obtain a near-complete highly divergent picobirnavirus genome from patient VS6600008. | ||
| 4 | Real-time PCR primers (VS791, VS792, VS793) labeled with 6-carboxy-fluorescein and 6-carboxy-tetramethyl-rhodamine | Performed on the total sample set to specifically detect sequences from picobirnavirus VS6600008. | ||
| 5 | Rolling circle amplification | Used to obtain a complete circular virus genome sequence (2,836 nt) from patient VS6600022. | ||
| 6 | Real-time PCR primers (VS794, VS795, VS796) labeled with 6-carboxy-fluorescein and 6-carboxy-tetramethyl-rhodamine | Performed on the total sample set to specifically detect sequences from the novel circular DNA virus in patient VS6600022. |