Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV022149 | GenBank Accession | KM361522 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 5141 |
| Definition | Human bocavirus isolate CU66, partial genome. | Submission Date | 2014-08-15 |
| Strain Name | CU66 | Sampling Date | from 2010 to 2013 |
| Sampling Country | Thailand | Location | Thailand |
| Submitted By | Smits,S.L., Prachayangprecha,S., Schapendonk,C., Koopmans,M.P., Osterhaus,A.D.M.E., Schurch,A.C., Pas,S.D., van der Eijk,A.A., Poovorawan,Y. and Haagmans,B.L. | Submitting Institution | Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands |
| Original Host | Homo sapiens | Sample Type | nasopharyngeal aspirates samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | OmniCleave endonuclease (Epicentre; Illumina) | Treatment of samples to deplete host nucleic acids. | 25100822 | View Related Publication |
| 2 | NucleoSpin RNA XS kit (Macherey-Nagel) | Extraction of RNA from the samples. | ||
| 3 | High Pure viral nucleic acids kit (Roche) | Isolation of viral nucleic acids. | ||
| 4 | MinElute PCR purification kit (Qiagen) | Purification of PCR products. | ||
| 5 | GS FLX Titanium rapid library MID adaptor kit (454 Life Sciences; Roche) | Addition of unique sequence tags to PCR products. | ||
| 6 | GS FLX Titanium library preparation kit (454 Life Sciences; Roche) | Preparation of a library of DNA fragments. | ||
| 7 | 454 GS Junior instrument (454 Life Sciences) | Sequencing of the prepared DNA libraries. | ||
| 8 | MagNA Pure LC total nucleic acid isolation kit and MagNA Pure LC isolation station (Roche) | Extraction of total nucleic acid from samples for real-time RT-PCR assays. |