Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV022081 GenBank Accession MK321698
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 555
Definition Human parvovirus B19 strain SM390 NS1 (NS1) and VP1 (VP1) genes, partial cds. Submission Date 2018-12-20
Strain Name SM390 Sampling Date from 2016-12 to 2017-01
Sampling Country Brazil Location Santa Maria city (extreme Brazilian South, Rio Grande do Sul State, 29° 41' 02" S, 53° 48' 25" E), Brazil
Submitted By Slavov,S.N., Rodrigues,E.S., Sauvage,V., Caro,V., Diefenbach,C.F., Zimmermann,A.M., Covas,D.T., Laperche,S. and Kashima,S. Submitting Institution Blood Center of Ribeirao Preto, University of Sao Paulo, Rua Tenente Catao Roxo 2501, Ribeirao Preto, Sao Paulo 14051-140, Brasil
Original Host Homo sapiens Sample Type blood samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 EDTA sterile vacutainers (Vacuette, Greiner Bio-One, Americana, So Paulo, Brazil) Blood collection 30851123 View Related Publication
2 Architect HIV Ag/Ab Combo, Abbott Serological testing for HIV
3 Architect anti-HCV kit, Abbott Serological testing for HCV
4 Architect anti-HBc II and Architect HBsAg II Qualitative, Abbott Serological testing for HBV
5 Architect rHTLV-I/II, Abbott Serological testing for HTLV-1/2
6 Rapid Plasm Reagin, RPR, Laborclin, Brazil Serological testing for Syphilis
7 Gold ELISA Chagas Kit, REM, Brazil Serological testing for Chagas disease
8 NAT HIV/HCV/HBV Kit (Bio-Manguinhos, Brazil) Testing for HIV, HCV RNA, and HBV DNA
9 Anti-Parvovirus B19 ELISA IgG kit (Euroimmun Medizinische Labordiagnostika AG, Lübeck, Germany) Testing for anti-B19V IgG to evaluate B19V seroprevalence
10 PureLink Viral RNA/DNA Mini Kit (ThermoFisher Scientific, So Paulo, Brazil) Extraction of viral DNA
11 TaqMan Universal Master Mix (ThermoFisher Scientific, So Paulo, Brazil) Component of the real-time PCR reaction mix for B19V DNA detection and quantification
12 pCR 2.1 TOPO TA Cloning vector (ThermoFisher Scientific, So Paulo, Brazil) Generation of standard curve for B19V DNA quantification
13 BigDyeTM Terminator v 3.1 Cycle Sequencing Kit (ThermoFisher Scientific, So Paulo, Brazil) Sequencing of B19V amplicons