Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV022070 GenBank Accession JX023433
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 372
Definition Human parvovirus B19 isolate AF216-9 NS1 (NS1) gene, partial cds. Submission Date 2012-05-04
Strain Name AF216-9 Sampling Date from 2010-03 to 2011-05
Sampling Country Brazil Location Hemotherapy Centre of Ribeira˜o Preto, city of Ribeira˜o Preto, Brazil (Hemocentro RP), Brazil
Submitted By Slavov,S.N., Kashima,S., Silva-Pinto,A.C., Amarilla,A.A., Alfonso,H.L., Aquino,V.H. and Covas,D.T. Submitting Institution Laboratory of Molecular Virology, Hemotherapy Center of Ribeirao Preto, Faculty of Medicine in Ribeirao Preto, USP, Rua Tenente Catao Roxo 2501, Ribeirao Preto, SP 14050230, Brasil
Original Host Homo sapiens Sample Type blood samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Ethylenediaminetetracetic acid (EDTA) tubes (Becton Dickinson, Franklin Lakes, NJ) For collecting blood samples by brachial vein puncture. 22930515 View Related Publication
2 Ridascreen Parvovirus IgG kit (R-Biopharm AG, Darmstadt, Germany) For the semi-quantitative detection of anti-B19V IgG antibodies.
3 Auto Reader II spectrophotometer (Ortho Clinical Diagnostic Systems, Raritan, NJ) For reading the colorimetric reaction at wavelengths of 420/630 nm.
4 QIAamp DNA blood mini kit (QIAGEN, Hilden, Germany) For DNA extraction from plasma samples.
5 In-house optimized TaqMan platform For quantitating viral load using specific primers and probe.
6 ABI Prism 7500 system (Life Technologies) For performing the quantitation of the viral load.
7 Platinum Taq DNA high fidelity polymerase (Life Technologies) For reducing the probability of introducing sequencing errors in PCR.
8 ABI3500XL genetic analyzer (Life Technologies), Big Dye Terminator Cycle Sequencing Kit v. 3.1 (Life Technologies) For performing sequencing of PCR-amplified products.
9 pCR 2.1-TOPO vector (Life Technologies) For cloning the 699 bp VP1 fragment and subsequent sequencing.
10 S.N.A.P. MiniPrep Kit (Life Technologies) For DNA extraction from bacterial colonies.