Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV022032 | GenBank Accession | EF590138 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 289 |
| Definition | Human parvovirus B19 isolate BT056 nonstructural protein 1 (NS1) gene, partial cds. | Submission Date | 2007-05-02 |
| Strain Name | BT056 | Sampling Date | from 2001-06 to 2002-05 |
| Sampling Country | Thailand | Location | thalassemia clinic, Siriraj Hospital, Thailand, Thailand |
| Submitted By | Siritantikorn,S., Kaewrawang,S., Siritanaratkul,N., Thongmee,C., Theamboonlers,A., Poovorawan,Y., Kantakamalakul,W. and Wasi,C. | Submitting Institution | Microbiology, Mahidol University, Bangkoknoi, Bangkok, Thailand |
| Original Host | None | Sample Type | blood samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | EDTA-blood | Collected to obtain plasma and peripheral blood mononuclear cells (PBMC) from thalassemic patients. | 18035805 | View Related Publication |
| 2 | Indirect ELISA kit (Genzyme Virotech GmbH, Germany) | Used to determine anti-parvovirus B19 IgG. | ||
| 3 | QIAamp DNA Blood Mini kit (CA, USA) | Used for DNA extraction from plasma and PBMC samples. | ||
| 4 | Nested PCR primers | Used to amplify the NS1 region of parvovirus B19 DNA. The sequences were given by Dr. Neal S. Young and Dr. Kevin E. Brown, Virus Discovery Group, Hematology Branch, National Heart, Lung and Blood Institute, NIH, USA. | ||
| 5 | Human parvovirus B19 DNA serum | Used as a positive control (PC) during PCR amplification. |