Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV021981 | GenBank Accession | KY906244 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 467 |
| Definition | Human bocavirus isolate BRSP72708 VP1 gene, partial cds. | Submission Date | 2017-04-05 |
| Strain Name | BRSP72708 | Sampling Date | 2010 |
| Sampling Country | Brazil | Location | São Paulo metropolitan area, Brazil |
| Submitted By | Silva,P.E., Figueiredo,C.A., Paiva,T.M., Afonso,A.M.S. and Oliveira,M.I. | Submitting Institution | Virology, Adolfo Lutz Institute, Av Dr Arnaldo 355, Sao Paulo, Sao Paulo 01246902, Brazil |
| Original Host | Homo sapiens | Sample Type | nasopharyngeal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAampViral DNA Mini kit | Extraction of Viral DNA | 29392492 | View Related Publication |
| 2 | qPCR for NS1 and NP-1 genes | Detection of Human Bocavirus (HBoV) | ||
| 3 | qRT-PCR for the human ribonuclease (RNase) P gene | Control for PCR inhibitors and monitor nucleic acid extraction efficiency | ||
| 4 | PCR targeting VP1/2 capsid gene | Amplification of HBoV positive samples | ||
| 5 | BigDyeTM kit v3.1 (Applied Biosystems, Inc.) | Sequencing of PCR amplicons | ||
| 6 | ABI 3130 sequencer (Applied Biosystems, Inc.) | Sequencing of dye-labeled products |