1
Universal transport medium (Copan, Brescia, Italy)
To collect and store nasopharyngeal swabs at ?80°C until use.
38051050
2
QIAamp 96 Virus QIAcube HT Kit (Qiagen, Hilden, Germany)
For the extraction of nucleic acids from specimens.
3
QIAamp Viral RNA Mini Kit (Qiagen)
For the extraction of viral RNA from specimens.
4
NucleoSpin 96 Virus (Macherey-Nagel, Duren, Germany)
For the extraction of viral RNA from specimens.
5
SMART MMLV Reverse Transcriptase (Takara-Bio, Shiga, Japan)
To synthesize cDNA from RNA using Oligo(dT) primer.
6
Oligo(dT) primer (Qiagen)
To synthesize cDNA from RNA.
7
Primer random (Roche)
To synthesize cDNA from RNA.
8
AgPath-ID One-Step RT-PCR reagents
For one-step real-time RT-PCR.
9
LightCycler 480 Probes Master
For two-step real-time PCR.
10
Primer/probe sets
To detect 17 respiratory viruses by real-time PCR and RT-PCR.
11
HBTEC (FC-0035, LIFELINE Cell Technology, Frederick, MD, USA)
To prepare air-liquid interface culture for respiratory virus isolation.
12
Traswell (3470, Corning, One Riverfront Plaza, NY, USA)
To culture HBTEC cells.
13
Differentiation medium
To culture human airway epithelium cells at an air-liquid interface.
14
1% fetal calf serum (FCS)-Dulbecco's modified Eagle's medium (DMEM)
To dilute specimens and wash cell cultures during virus isolation.
15
Penicillin-streptomycin, gentamicin, and fungizone
To prevent bacterial and fungal contamination in cell cultures.
16
MC-210 (KAC Co Ltd., Hyogo, Japan)
To remove mycoplasmas from the basolateral medium.
17
Quantus Fluorometer (Promega, Madison, WI, USA)
To measure fluorescence and determine viral copy numbers.
18
NEBNext Ultra II RNA Library Prep Kit for Illumina [New England Biolabs (NEB), Ipswich, MA, USA]
To prepare RNA libraries for next-generation sequencing.
19
NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB)
To prepare DNA libraries for next-generation sequencing.
