Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV021799 GenBank Accession ON756064
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate2 Sequence Length 521
Definition Bocaparvovirus sp. isolate BoV/Human-wt/CHN/19161076/2019/BoV-2 capsid protein gene, partial cds. Submission Date 2022-06-12
Strain Name BoV/Human-wt/CHN/19161076/2019/BoV-2 Sampling Date 2019-02
Sampling Country China Location Chaoyang district of the urban area, Tongzhou district of the suburbs, and Huairou district of the outer suburbs, China
Submitted By Shen,L., Yan,H., Li,W. and Gao,Z. Submitting Institution Institute of Infectious Diseases and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, No. 16, Hepingli Middle Street, Beijing, Dongcheng District 100013, China
Original Host Homo sapiens Sample Type fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp Viral RNA Mini Kit Used for viral RNA extraction from fecal suspension samples. 36713165 View Related Publication
2 Real-time RT-PCR kits (Jiangsu Uninovo Biological Technology Co., Ltd.) Used to detect respiratory viruses in fecal samples.
3 Real-time PCR kits (Applied Biological Technologies Co., Ltd.) Used to detect severe acute respiratory SARS-CoV-2.
4 TaqManTM Microbial Array Specialty Card (Thermo Fisher Scientific Co., Ltd.) Used to detect a panel of diarrhea bacteria and parasites.
5 QIAGEN One-Step RT-PCR Kit Used for amplifying HRV VP4/2 region by nested PCR.
6 AmpliTaq GoldTM 360 Master Mix (Thermo Fisher Scientific Co., Ltd.) Used for the second-round PCR of HRV VP4/2 region and for amplifying BoV VP1/2 region.
7 Hi-Hela cells Used for HRV isolation and detection of cytopathic effect.
8 Artificial gastric acid Used for digesting clinical specimens and virus nucleic acid. Prepared with hydrochloric acid, pepsin, and NaCl.
9 Artificial bile/pancreatic juice Used for digesting clinical specimens and virus nucleic acid. Contains PBS, ox bile, and pancreatin.