Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV021776 | GenBank Accession | KU871314 |
| Subfamily | Parvovirinae | Genus | Tetraparvovirus |
| Species | Tetraparvovirus primate1 | Sequence Length | 5160 |
| Definition | Human parvovirus 4 isolate K172C, complete genome. | Submission Date | 2016-03-03 |
| Strain Name | K172C | Sampling Date | |
| Sampling Country | South Africa | Location | Durban and Kimberley (South Africa) and Gaborone (Botswana), South Africa |
| Submitted By | Sharp,C.P., Gregory,W.F., Malik,A., Jooste,P., Ndung'u,T., Ogwu,A., Shapiro,R., Riddell,L., Chen,F., Luzzi,G., Adland,E., Goulder,P.J.R., Simmonds,P., Klenerman,P. and Matthews,P.C. | Submitting Institution | Infection and Immunity, The Roslin Institute, University of Edinburgh, The Roslin Institute Building, Easter Bush Campus, Edinburgh, Midlothian EH25 9RG, Scotland |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Superscript III reverse transcriptase | For synthesizing cDNA from RNA samples. | 28497124 | View Related Publication |
| 2 | RNeasy mini kit (Qiagen) | For extracting RNA from pooled serum samples. | ||
| 3 | GoTaq DNA polymerase (Promega) | For performing PCR reactions. | ||
| 4 | DNeasy blood and tissue kit (Qiagen) | For extracting DNA from pooled serum samples. | ||
| 5 | BigDye Terminator v3.1 (Applied Biosystems) | For performing direct amplicon sequencing for PARV4. | ||
| 6 | Biokit enzyme immune assay (Barcelona, Spain) | For determining HBsAg status. | ||
| 7 | Murex HBsAg v3 (DiaSorin) | For detecting HBsAg. | ||
| 8 | PCR-sequence specific primer typing | For obtaining high resolution HLA Class I data. | ||
| 9 | BAC TO GG | Indirect ELISA for PARV4 IgG screening. | ||
| 10 | Roche Amplicor Version 1.5 assay (Rotkreuz, Switzerland) or Abbott Laboratories m2000 platform (Abbott Park, IL, USA) | For determining HIV-1 RNA viral load. | ||
| 11 | Cryopreserved PBMCs | For screening ex vivo CD8+ T cell responses using IFN-gamma ELISpot assays. | ||
| 12 | IFN-gamma ELISpot assays | For quantifying IFN-gamma responses to PARV4 peptides. | ||
| 13 | Schafer-N peptides | For synthesizing truncations of PARV4 epitopes for IFN-gamma ELISpot assays. | ||
| 14 | PBMC ELISpot media | For maintaining PBMCs during ELISpot assays. |