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1
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Paraffin-embedded tissue samples
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Provided as experimental samples from the archives for analysis.
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28285965
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2
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Xylene
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Used to deparaffinize tissue sections.
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3
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Digestion buffer containing proteinase K
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Used to digest tissue sections post-deparaffinization.
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4
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Phenol/chloroform
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Used for DNA extraction from tissue samples.
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5
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PCR primers for human β-globin gene
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Used to evaluate the quality of extracted DNA.
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6
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Outer PCR primers (MY09/MY11)
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Consensus primer pair used in the first round of nested PCR for HPV detection targeting the HPV L1 gene.
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7
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Inner PCR primers (GP5+/GP6+)
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Consensus primer pair used in the second round of nested PCR for HPV detection targeting the HPV L1 gene.
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8
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AAV primers F1, Ra, Rb (first round), F2, Ra, Rb (second round)
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Designed for semi-nested PCR to detect AAV (Rep gene).
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9
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PUC57 plasmid enclosing desired fragment
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Used as the positive control for AAV DNA amplification.
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10
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Positive cervical sample
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Used as the positive control for HPV DNA amplification.
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