Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV021426 GenBank Accession M13178
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 5112
Definition Human parvovirus B19-Au DNA, partial genome. Submission Date 1995-05-17
Strain Name None Sampling Date
Sampling Country None Location None
Submitted By Shade,R.O., Blundell,M.C., Cotmore,S.F., Tattersall,P. and Astell,C.R. Submitting Institution J. Virol. 58 (3), 921-936 (1986)
Original Host None Sample Type serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Restriction endonucleases (EcoRI, BamHI, AvaI, BglII, Clal, EcoRV, HindIII, PstI, PvuI, PvuII, Sall, SstI, SstII, XbaI, and XhoI) Used for digesting DNA for mapping and sequencing 3701931 View Related Publication
2 T4 DNA ligase Used for ligating DNA fragments
3 T4 polynucleotide kinase Used for labeling DNA ends
4 DNA polymerase I (Klenow fragment) Used for DNA synthesis and filling-in reactions
5 Exonuclease III Used for generating nested deletions
6 Linkers Used for attaching specific sequences to DNA ends for cloning
7 Si nuclease Used for removing single-stranded tails
8 α-32P-deoxyribonucleoside triphosphates (dNTPs) Used for DNA sequencing
9 α-Thio-dNTPs (αS-dNTPs) Used for generating protected DNA ends in sequencing
10 NACS-52 columns Used for DNA purification
11 Alkaline lysis solutions Used for plasmid DNA isolation
12 CsCl density gradient Used for plasmid DNA purification
13 Phenol-CHCl3 Used for DNA extraction and purification
14 Ethanol Used for DNA precipitation
15 Synthetic deoxyribooligonucleotides (13- to 16-mers) Used as primers for DNA sequencing