Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV021408 GenBank Accession AY083236
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 994
Definition B19 virus isolate CDC156 NS1 and VP1u genes, partial cds. Submission Date 2002-03-11
Strain Name CDC156 Sampling Date from 1972 to 2001-09
Sampling Country None Location None
Submitted By Servant,A., Laperche,S., Lallemand,F., Marhino,V., De Saint Maur,G., Meritet,J.F. and Garbarg-Chenon,A. Submitting Institution Laboratoire de Virologie, Hopital Trousseau (EA2391, UFR Saint-Antoine), 26 Avenue du Dr. Arnold Netter, Paris 75012, France
Original Host None Sample Type serum samples,bone marrow samples,amniotic fluid or fetal blood samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp DNA minikit (Qiagen, Courtaboeuf, France) DNA extraction from serum samples. 12186896 View Related Publication
2 parvovirus B19 immunoglobulin G (IgG) or IgM enzyme immunoassay [third generation]; Biotrin, Dublin, Ireland Testing for B19 virus-specific antibodies.
3 AmplitaqGold (Applied Biosystems, Villebon, France) DNA polymerase used in PCR.
4 New England Biolabs, Ozyme, St. Quentin en Yvelines, France Supplies MfeI and ApaI enzymes for restriction length polymorphism analysis.
5 Expand High Fidelity PCR system (Roche Molecular Biochemicals, Meylan, France) PCR amplification of large DNA sequences.
6 EcoRI (enzyme tail) Enzyme restriction site included for cloning purposes.
7 QIAquick gel extraction and QIAquick PCR purification kits (Qiagen) Puri?cation of PCR products from gel slices.
8 ABI Prism 377 automatic sequencer (Applied Biosystems) Automated DNA sequencing.
9 ABI PRISM DyeTerminator cycle sequencing Ready Reaction kit (Applied Biosystems) Cycle sequencing of DNA.
10 Invitrogen, Groningen, The Netherlands Supplies pcDNAII and pcDNA3.1 HisB plasmids for DNA cloning.
11 Escherichia coli TOP10F’(Invitrogen) Bacterial host for plasmid propagation.