Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV021365 GenBank Accession EU144349
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 1081
Definition Human parvovirus B19 isolate BN47 non-structural protein NS1 (NS1) and minor capsid protein VP1 (VP1) genes, partial cds. Submission Date 2007-09-10
Strain Name BN47 Sampling Date
Sampling Country Germany Location Germany
Submitted By Schneider,B., Hone,A., Tolba,R.H., Fischer,H.-P., Blumel,J. and Eis-Hubinger,A.M. Submitting Institution Institute of Medical Microbiology, Immunology and Parasitology, University of Bonn, Sigmund-Freud-Str. 25, Bonn 53105, Germany
Original Host None Sample Type liver specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Baculovirus-expressed VP2 ELISA kit (Biotrin) To measure anti-B19V IgG and IgM antibodies in sera. 18089740 View Related Publication
2 Spin-column DNA preparation procedure To prepare DNA from liver tissue.
3 Generic nested PCR kit For the detection of genotype 1 DNA.
4 Generic nested PCR kit For the detection of genotype 2 DNA.
5 Custom primers (Outer forward, inner forward, outer reverse, inner reverse) For the amplification of genotype 3 DNA.
6 Expand High Fidelity PCR system (Roche; enzyme A) For PCR amplification.
7 Easy-A High-Fidelity PCR Cloning Enzyme (Stratagene; enzyme B) For PCR amplification with high fidelity.
8 BD Advantage-HF 2 kit (BD Biosciences Clontech; enzyme C) For PCR amplification with high fidelity.
9 PfuUltra High-Fidelity DNA Polymerase (Stratagene; enzyme D) For PCR amplification with high fidelity.
10 QIAquick PCR Purification kit (Qiagen) To purify nested PCR products.
11 ABI Prism BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) For sequencing reactions.
12 ABI Prism 3130 (Applied Biosystems) To run sequencing reactions.
13 Sterile pharmacy water To dilute nucleic acid preparation.
14 Plasmid pGEM-1/B19 To generate genotype 1 PCR products via plasmid template.
15 Plasmid pCR4-TOPO (Invitrogen) To clone PCR products.
16 Plasmid template (pDV-IIIG1, pDV-IIIG2) For validation experiments involving mixing experiments with cloned plasmids.