Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV021309 GenBank Accession EU175863
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 115
Definition Human parvovirus 4 strain BONN-6 ORF1 gene, partial cds. Submission Date 2007-09-20
Strain Name BONN-6 Sampling Date
Sampling Country Germany Location Germany
Submitted By Schneider,B., Fryer,J.F., Reber,U., Fischer,H.P., Tolba,R.H., Baylis,S.A. and Eis-Hubinger,A.M. Submitting Institution Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, Bonn 53105, Germany
Original Host None Sample Type liver specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Liver specimens Obtained from various sources to investigate the presence of PARV4 DNA using PCR. 18098166 View Related Publication
2 Bone marrow specimens Collected from autopsied individuals to test for the presence of PARV4 DNA.
3 Serum samples Collected at or shortly before tissue collection to test for the presence of PARV4 DNA.
4 DNA extraction reagents To extract DNA from specimens for PCR analysis.
5 Primers (specific sequences listed) Used in nested PCR and sequencing reactions to amplify specific regions of PARV4 DNA.
6 PCR amplicon Generated by outer primers and cloned into the pCR14 TOPO vector for sensitivity determination.
7 pCR14 TOPO vector (Invitrogen, Karlsruhe, Germany) Used for cloning PCR amplicons to measure PCR sensitivity.
8 ABI PRISM1 BigDye1 Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Darmstadt, Germany) Used to perform sequencing reactions.
9 AmpliTaq Gold1 DNA polymerase Enzyme used in sequencing reactions.
10 ABI PRISM1 3130 instrument (Applied Biosystems) Equipment used to run sequencing reactions.
11 Real-time PCR reagents Used to determine PARV4 DNA loads in tissues using a consensus sequence real-time PCR.
12 Plasmid DNA containing a short fragment of PARV4 DNA Used to generate a standard curve for real-time PCR assays.