Panoramic Parvovirus Strain Discovery Data Chain

Download Strain's Sequence
Feedback for This Record
ParvoDB ID PV021303 GenBank Accession EU175857
Subfamily Parvovirinae Genus Tetraparvovirus
Species Tetraparvovirus primate1 Sequence Length 3171
Definition Human parvovirus 4 strain BONN-14 ORF1 and ORF2 genes, partial cds. Submission Date 2007-09-19
Strain Name BONN-14 Sampling Date
Sampling Country Germany Location Germany
Submitted By Schneider,B., Fryer,J.F., Reber,U., Fischer,H.P., Tolba,R.H., Baylis,S.A. and Eis-Hubinger,A.M. Submitting Institution Institute of Virology, University of Bonn Medical Centre, Sigmund-Freud-Str. 25, Bonn 53105, Germany
Original Host None Sample Type liver specimens
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Liver specimens Obtained from various sources to investigate the presence of PARV4 DNA using PCR. 18098166 View Related Publication
2 Bone marrow specimens Collected from autopsied individuals to test for the presence of PARV4 DNA.
3 Serum samples Collected at or shortly before tissue collection to test for the presence of PARV4 DNA.
4 DNA extraction reagents To extract DNA from specimens for PCR analysis.
5 Primers (specific sequences listed) Used in nested PCR and sequencing reactions to amplify specific regions of PARV4 DNA.
6 PCR amplicon Generated by outer primers and cloned into the pCR14 TOPO vector for sensitivity determination.
7 pCR14 TOPO vector (Invitrogen, Karlsruhe, Germany) Used for cloning PCR amplicons to measure PCR sensitivity.
8 ABI PRISM1 BigDye1 Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Darmstadt, Germany) Used to perform sequencing reactions.
9 AmpliTaq Gold1 DNA polymerase Enzyme used in sequencing reactions.
10 ABI PRISM1 3130 instrument (Applied Biosystems) Equipment used to run sequencing reactions.
11 Real-time PCR reagents Used to determine PARV4 DNA loads in tissues using a consensus sequence real-time PCR.
12 Plasmid DNA containing a short fragment of PARV4 DNA Used to generate a standard curve for real-time PCR assays.