ParvoDB-Parvovirus Database

ParvoDB ID	PV021261	GenBank Accession	OP255990
Subfamily	Parvovirinae	Genus	Bocaparvovirus
Species	Bocaparvovirus primate1	Sequence Length	5464
Definition	Human bocavirus 1 strain R04-014/Hu/NL/2018, complete genome.	Submission Date	2022-08-12
Strain Name	R04-014/Hu/NL/2018	Sampling Date	2018-12-21
Sampling Country	Netherlands	Location	medical microbiology laboratories, Netherlands
Submitted By	Schmitz,D., Zwagemaker,F., van der Veer,B., Vennema,H., Bosch,T., Laros,J.F.J., Koopmans,M.P.G., de Graaf,M. and Kroneman,A.	Submitting Institution	Center for Infectious Diseases, Rijksinstituut voor Volksgezondheid en Milieu, Antonie van Leeuwenhoeklaan 9, Bilthoven, Utrecht 3721 MA, Netherlands

Number	Reagent/Consumables	Purpose	PMID/Url	Reference
1	Modified Eagle medium (MEM)	To prepare feces suspensions for nucleic acid extraction.	37432120	View Related Publication
2	Gentamicin	To prevent bacterial contamination in feces suspensions.
3	Costar Spin-X 0.45-μm CA membrane centrifuge tube filters (Corning)	To filter supernatant of feces suspensions before nuclease treatment.
4	MgCl2	To supplement filtrate for nuclease treatment.
5	OmniCleave endonuclease (EpiCentre)	To degrade non-viral nucleic acids in the sample.
6	MagNA Pure 96 system (Roche)	For total nucleic acid extraction from feces samples.
7	SuperScript III (Invitrogen)	To synthesize first-strand cDNA from extracted RNA.
8	NEBNext mRNA second-strand synthesis module (New England Biolabs)	To synthesize second cDNA strand.
9	DNA Clean & Concentrator-5 capped columns (Zymo Research)	To purify double-stranded DNA.
10	Nextera XT DNA library prep kit (Illumina)	To prepare DNA library for sequencing.
11	Illumina NextSeq instrument	For 150-nucleotide paired-end sequencing.
12	Illumina MiSeq instrument	For 300-nucleotide paired-end sequencing.

Panoramic Parvovirus Strain Discovery Data Chain