|
1
|
VP2 IgM EIA (Biotrin, Dublin, Ireland)
|
Measurement of VP2-specific IgM antibodies in serum samples.
|
16332455
|
View Related Publication
|
|
2
|
Light-Cycler-based B19-DNA PCR (Roche Diagnostics GmbH, Mannheim, Germany)
|
Semi-quantitative detection and quantification of B19 DNA in serum samples.
|
|
3
|
MagNA Pure LC automated instrument (Roche Diagnostics GmbH, Mannheim, Germany)
|
Extraction of nucleic acids for PCR assays.
|
|
4
|
LC FastStart DNA Master Hyb Probes kit (Roche Diagnostics GmbH, Mannheim, Germany)
|
Preparation of PCR master mixes containing FastStart Taq-Polymerase and dNTP-mix for DNA amplification.
|
|
5
|
Uracil-N-glycosylase (UNG)
|
Inclusion in PCR master mix to prevent carry-over contamination.
|
|
6
|
ABI Big Dye Terminator Cycle Sequencing Kit v2.0 (ABI, Germany)
|
DNA sequencing of PCR products.
|
|
7
|
VP1-IgG avidity EIA
|
Measurement of IgG avidity to determine the timing of B19 infection.
|
|
8
|
VP2-IgG-ETS EIA (prototype)
|
Detection of epitope-type specific VP2 IgG to diagnose acute-phase B19 infection.
|
|
9
|
Synthetic peptide containing the VP2 epitope (KYVTGIN)
|
Antigen used in the ETS-EIA for detection of acute-phase specific antibodies.
|
|
10
|
VP2 capsids
|
Used as the antigen in quantitative IgG EIA for detection of VP2-specific IgG antibodies.
|
|
11
|
Four-branched KYVTGIN peptide (New England Peptide, Gardner, MA)
|
Variant antigen used in ETS-EIA for improved detection of acute-phase specific antibodies.
|
|
12
|
Recombinant VP2 capsids
|
Antigen used in the in-house EIA for detection of VP2-specific IgG antibodies.
|
|
13
|
Ethanol
|
Used for precipitation of unincorporated dye terminators in DNA sequencing.
|
