1
NucliSens Magnetic Extraction Reagent
For DNA extraction.
20382557
2
BigDye Terminator v3.1 Cycle Sequencing Kit
For sequencing DNA.
3
3130xl Genetic Analyzer
For DNA sequencing.
4
Sucrose cushion
Used for ultracentrifugation to concentrate HBoV3-positive stool specimen before negative stain electron microscopy.
5
FEI Technai BioTwinR transmission electron microscope
For viewing negatively stained electron microscopy specimens.
6
Ammonium molybdate–trehalose solution (5% ammonium molybdate–1% trehalose in water, pH 6.9)
Used as a stain for negative stain electron microscopy.
7
MgCl2
Used in PCR reaction at a final concentration of 3 mM.
8
Primers P1 (CAT ATT ATA GTT GGG GGA GAA GG) and P2 (GGT AGT TTT TGA AGA AGC GAA GAG)
To amplify a 286 bp fragment of both HBoV1 and HBoV3.
9
Primers P5 (TCA GAA GCA TCG GAA GTG GGT GTT) and P6 (ATG TGA GGC TTT ATG CTG GCT GAA)
To amplify a 440 bp fragment of only HBoV3.
10
Agarose gel
For electrophoresis to detect PCR products.
11
Ethidium bromide
For staining in agarose gel electrophoresis.
12
Nested-PCR reactions
For testing HBoV2 in stool samples.
