Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV021135 | GenBank Accession | GU256650 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate2 | Sequence Length | 370 |
| Definition | Human bocavirus isolate RC4 nonstructural protein NS1 gene, partial cds. | Submission Date | 2009-12-02 |
| Strain Name | RC4 | Sampling Date | 2006-09-04 |
| Sampling Country | Brazil | Location | Brazil |
| Submitted By | Santos,N., Peret,T., Humphrey,C.D., Albuquerque,M.C., Silva,R.C., Benati,F.J., Lu,X. and Erdman,D. | Submitting Institution | Virology, UFRJ, Cidade Universitaria - Ilha Do Fundao, Rio de Janeiro, Rio de Janeiro 21941-590, Brazil |
| Original Host | Homo sapiens | Sample Type | stool samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | NucliSens Magnetic Extraction Reagent | For DNA extraction. | 20382557 | View Related Publication |
| 2 | BigDye Terminator v3.1 Cycle Sequencing Kit | For sequencing DNA. | ||
| 3 | 3130xl Genetic Analyzer | For DNA sequencing. | ||
| 4 | Sucrose cushion | Used for ultracentrifugation to concentrate HBoV3-positive stool specimen before negative stain electron microscopy. | ||
| 5 | FEI Technai BioTwinR transmission electron microscope | For viewing negatively stained electron microscopy specimens. | ||
| 6 | Ammonium molybdate–trehalose solution (5% ammonium molybdate–1% trehalose in water, pH 6.9) | Used as a stain for negative stain electron microscopy. | ||
| 7 | MgCl2 | Used in PCR reaction at a final concentration of 3 mM. | ||
| 8 | Primers P1 (CAT ATT ATA GTT GGG GGA GAA GG) and P2 (GGT AGT TTT TGA AGA AGC GAA GAG) | To amplify a 286 bp fragment of both HBoV1 and HBoV3. | ||
| 9 | Primers P5 (TCA GAA GCA TCG GAA GTG GGT GTT) and P6 (ATG TGA GGC TTT ATG CTG GCT GAA) | To amplify a 440 bp fragment of only HBoV3. | ||
| 10 | Agarose gel | For electrophoresis to detect PCR products. | ||
| 11 | Ethidium bromide | For staining in agarose gel electrophoresis. | ||
| 12 | Nested-PCR reactions | For testing HBoV2 in stool samples. |