Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV021128 | GenBank Accession | OR123962 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 5391 |
| Definition | Erythroparvovirus primate1 isolate CD1_B19V_2019 nonstructural protein NS1 (NS), 7.5 kDa protein, minor capsid protein (VP1), major capsid protein (VP2), and small nonstructural protein genes, complete cds. | Submission Date | 2023-06-10 |
| Strain Name | CD1_B19V_2019 | Sampling Date | from 2019-01 to 2019-12 |
| Sampling Country | Brazil | Location | Brazil |
| Submitted By | Santos,M.A.M., Pavon,J.A.R., Viniski,A.E., Souza,C.L.C., Oliveira,E.C., Medeiros,D.B.A., Silva,S.P., Nunes,M.R.T. and Slhessarenko,R.D. | Submitting Institution | Faculdade de Medicina, Universidade Federal de Mato Grosso, AV. Fernando Correa da Costa, 2367, Cuiaba, Mato Grosso 78060-900, Brasil |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | MagMax viral pathogen nucleic acid isolation kit (Applied Biossystems) | Used for viral nucleic acid extraction from serum samples. | 37544570 | View Related Publication |
| 2 | SuperScriptTM VILOTM MasterMix | Used for converting viral nucleic acid to double-strand cDNA (ds-cDNA). | ||
| 3 | NEBNext Second Strand Synthesis Module | Used for synthesizing the second strand of cDNA. | ||
| 4 | PureLink PCR Purification Kit (Invitrogen) | Used for purification of ds-cDNA. | ||
| 5 | Qubit Assay DNA HS Kit (Qubit 4.0, Invitrogen) | Used for quantification of ds-cDNA. | ||
| 6 | Nextera XT DNA (Illumina Inc) | Used for library synthesis of ds-cDNA. | ||
| 7 | NextSeq 500/550 High Output Kit v2.5 (300 Cycles) (Illumina, Inc) | Used for paired-end sequencing on NextSeq 500. | ||
| 8 | Purelink viral RNA/DNA kit (Invitrogen, USA) | Used for viral RNA extraction from individual serum samples. | ||
| 9 | Genus-specific primers | Used for converting viral RNA to cDNA. | ||
| 10 | GoScript reverse transcriptase (Promega) | Used for reverse transcription of viral RNA to cDNA. |