Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV021037 | GenBank Accession | DQ229360 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 389 |
| Definition | Human parvovirus B19 isolate 05BR0127 non-structural protein (NS1) gene, partial cds. | Submission Date | 2005-10-03 |
| Strain Name | 05BR0127 | Sampling Date | from 2003-12 to 2005-03 |
| Sampling Country | Brazil | Location | Brazil |
| Submitted By | Sanabani,S.S., Kleine,W.N., Pereira,J. and Sabino,E.C. | Submitting Institution | Molecular Biology, Fundacao Pro-Sangue, Hemocentro de Sao Paulo, Av. Dr. Eneas de Carvalho Aguiar, 155 1ST Floor, Sao Paulo 05403 000, Brazil |
| Original Host | None | Sample Type | bone marrow samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Qiagen Qiamp blood kit | Extraction of genomic DNA from bone marrow samples | 16455922 | View Related Publication |
| 2 | Tris (pH 8.3; Invitrogen, Brazil) | PCR reaction buffer component | ||
| 3 | MgCl2 | PCR reaction buffer component | ||
| 4 | PCR primers | Amplification of specific DNA fragments | ||
| 5 | Deoxynucleoside triphosphates | Substrates for DNA synthesis during PCR | ||
| 6 | Taq polymerase (Invitrogen, Brazil) | DNA synthesis during PCR | ||
| 7 | Viral quality control B19 DNA (VQC; Sanquin-CLB, Alkmaar, The Netherlands) standard | Evaluation of PCR detection sensitivity | ||
| 8 | Erythrovirus-negative genomic DNA (polymorphonuclear cells) | Negative control for PCR detection sensitivity | ||
| 9 | Agarose gel | Electrophoresis for analyzing amplified products | ||
| 10 | Ethidium bromide | Staining DNA fragments for visualization under UV illumination | ||
| 11 | Fluorescent-dye terminators | DNA sequencing reagents for automated sequencer |