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1
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Axygen extraction columns
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To obtain nucleic acids from serum samples.
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32974527
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2
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AxyPrep Body Fluid Viral DNA and RNA Purification Miniprep kit
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For the purification of viral DNA and RNA.
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3
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TE buffer
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To store extracted nucleic acids.
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4
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DMSO
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Reagent used in the PCR mixture for B19V detection.
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5
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MgCl2
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Reagent used in the PCR mixture for both B19V and HBoV1 detection.
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6
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Deoxynucleotides
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Reagent used in the PCR mixture for both B19V and HBoV1 detection.
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7
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Taq DNA polymerase
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Enzyme used in PCR for both B19V and HBoV1 detection.
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8
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PCR primers
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Used for the amplification of target DNA regions in B19V and HBoV1 detection.
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9
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Invitrogen/Thermo Fisher Scientific PCR reagents
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Reagents used for PCR, including Taq DNA polymerase and PCR primers.
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10
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Polyacrylamide gel (10%)
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For the electrophoresis separation of PCR products.
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11
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Ethanol
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For fixing polyacrylamide gels.
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12
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Acetic acid
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For fixing polyacrylamide gels.
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13
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AgNO3 (Silver nitrate)
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For staining polyacrylamide gels.
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14
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Sodium hydroxide
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For developing polyacrylamide gels.
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15
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Formol
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For developing polyacrylamide gels.
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16
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Qubit fluorometer
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For the quantification of PCR products.
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17
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Qiagen QIAquick PCR Purification kit
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For the purification of PCR products.
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18
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Ridascreen enzyme-linked immunosorbent assays
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For the determination of anti-B19V antibodies.
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19
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RIDA RF-Absorbens
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For the precipitation of IgG antibodies in anti-B19V IgM assay.
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