Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV020797 GenBank Accession OQ695614
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 546
Definition Human bocavirus 1 strain AC99/BRA/2012/HBoV-1 capsid protein VP1 and capsid protein VP2 genes, partial cds. Submission Date 2023-03-24
Strain Name AC99/BRA/2012/HBoV-1 Sampling Date from 2012-01 to 2012-12
Sampling Country Brazil Location Rio Branco, Acre, Brazil
Submitted By Trindade,F.D.B., Ramos,E.S.F., Guerra,S.F.S., Lobo,P.S., Cardoso,J.F., Penha,E.T. Jr., Bezerra,D.A.M., Kimura,M.A.O.N., Andrade,J.A.A., Silva,M.C.M., Mascarenhas,J.D.P. and Soares,L.S. Submitting Institution Virology, Evandro Chagas Institute, Rodovia BR-316 km 7 s/n - Levilandia, Ananindeua, PARA 67030-000, Brazil
Original Host Homo sapiens Sample Type stool samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 10% fecal suspensions with Tris-calcium buffer (pH = 7.2) To prepare samples for nucleic acid extraction. 37113004 View Related Publication
2 Silica glass powder To extract viral nucleic acids (DNA and RNA viruses) from fecal suspensions.
3 RNAse/DNAse-free water Used as a negative control in extraction procedures.
4 Primers for nested PCR targeting VP1/VP2 region To detect and amplify HBoV-specific sequences.
5 1.5% agarose gel To separate and visualize PCR products by electrophoresis.
6 Nucleic acid staining solution To stain the agarose gel for visualization of DNA bands.
7 ExoSAP-IT enzymes (Applied Biosystems) To purify PCR amplicons for sequencing.
8 BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems) To perform cycle sequencing of purified PCR products.