Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV020748 | GenBank Accession | KF193595 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 454 |
| Definition | Human bocavirus isolate 1176-HCM/3.11 VP1 gene, partial cds. | Submission Date | 2013-06-06 |
| Strain Name | 1176-HCM/3.11 | Sampling Date | from 2010-04 to 2011-05 |
| Sampling Country | Vietnam | Location | Ho Chi Minh City, Vietnam, Vietnam |
| Submitted By | Tran,D.N. | Submitting Institution | Developmetal Medical Sciences, The University of Tokyo, Bunkyo-ku Hongo 7-3-1, Tokyo 113-0033, Japan |
| Original Host | Homo sapiens | Sample Type | Nasopharyngeal flocked swabs |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Nasopharyngeal flocked swabs (MicroRheologics, Brescia, Italy) | To collect nasopharyngeal samples from enrolled children within 24 hours after admission. | 24123072 | View Related Publication |
| 2 | Sterile physiological saline fluid | To store nasopharyngeal specimens immediately after collection. | ||
| 3 | QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) | To extract viral genomes from the specimens. | ||
| 4 | Primers AK-VP-F1, AK-VP-R1, AK-VP-F2, AK-VP-R2 | To detect HBoV by PCR method and amplify the partial VP1 gene of HBoV. | ||
| 5 | Multiplex hemi-nested (RT)-PCR protocol | To screen samples for other respiratory viruses such as respiratory syncytial virus, influenza virus A and B, human metapneumovirus, parainfluenza virus type 1–4, human rhinoviruses, human coronaviruses, and adenovirus. |