ParvoDB-Parvovirus Database

ParvoDB ID	PV020734	GenBank Accession	KY050744
Subfamily	Parvovirinae	Genus	Bocaparvovirus
Species	Bocaparvovirus primate2	Sequence Length	4898
Definition	Human bocavirus 2 strain 03EI-02-10069/VietNam/2013, partial genome.	Submission Date	2016-10-26
Strain Name	03EI-02-10069/VietNam/2013	Sampling Date	2013-11-05
Sampling Country	Vietnam	Location	Vietnam
Submitted By	Tran Tan,T., Hoang Minh Tu,V., Nguyen Thi Thu,H., Le Nguyen Truc,N., Nguyen To,A., Ha Manh,T., Ho Van,H., Nguyen Manh,T., Trinh Trung,K., Truong Huu,K., Le Nguyen Thanh,N., Nguyen Thanh,H., Nguyen Van Vinh,C., Thwaites,G., van Doorn,H.R. and Le Van,T.	Submitting Institution	Emerging Infections, Oxford University Clinical Research Unit, 764 Vo Van Kiet, Ho Chi Minh +84, Vietnam

Number	Reagent/Consumables	Purpose	PMID/Url	Reference
1	Selected swabs	To collect clinical specimens for whole-genome sequencing.	28090592	View Related Publication
2	DNAse	To treat the samples and remove excess host DNA.
3	QIAamp viral RNA kit (QIAgen GmbH, Hilden, Germany)	To isolate viral nucleic acid (NA) from the supernatant.
4	Elution buffer (provided with the QIAamp kit)	To recover the isolated viral nucleic acid.
5	Super Script III kit (Invitrogen, Carlsbad, CA, USA)	To synthesize cDNA from the isolated viral nucleic acid.
6	FR26RV-Endoh primer	To prime the synthesis of cDNA.
7	exo-Klenow (Invitrogen)	To convert the cDNA to double-stranded DNA.
8	Platinum PCR supermix (Invitrogen)	To pre-amplify the double-stranded DNA.
9	FR20RV primer	Used in the pre-amplification of double-stranded DNA.
10	Nextera XT DNA sample preparation kit (Illumina, San Diego, CA, USA)	To prepare the PCR product for library preparation.
11	MiSeq reagent kits (Illumina)	To sequence the prepared DNA library on the Illumina MiSeq platform.

Panoramic Parvovirus Strain Discovery Data Chain