ParvoDB ID PV020711 GenBank Accession MT988401
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 5588
Definition Human parvovirus B19 isolate AA-18, partial genome. Submission Date 2020-09-08
Strain Name AA-18 Sampling Date 2015
Sampling Country Finland Location Finland
Submitted By Toppinen,M., Sajantila,A., Pratas,D., Hedman,K. and Perdomo,M.F. Submitting Institution Virology, University of Helsinki, Haartmaninkatu 3, Helsinki 00290, Finland
Original Host Homo sapiens Sample Type The BM from the diaphysis of femoral bone
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp DNA Mini Kit (Qiagen) DNA extraction from bone marrow samples 33968803 View Related Publication
2 Genomic DNA Reagent Kit Confirmation of the quality and integrity of total DNA in each sample
3 Single-use disposable plastics and filter tips Preventing contamination during experiments
4 Negative controls Ensuring accuracy and preventing contamination in experimental steps
5 Customized panel of biotinylated RNA oligonucleotides (Arbor Biosciences) Targeted enrichment of viral DNAs
6 KAPA HyperPlus library preparation kit (Roche) Preparation of sequencing libraries from sonicated total DNA
7 MyBaits v4 kit (Arbor Biosciences) Enrichment of viral genomes using hybridization on individual samples
8 Validated in-house quantitative PCRs Detection of B19V, MCPyV, TTV, and the nine HHVs
9 Hepatitis B Virus PCR Kit (GeneProof, Genesig) Testing for HBV viral DNA
10 Human papillomavirus 31 Standard kit (PrimerDesign) Testing for HPV-31 viral DNA
11 Human single-copy gene RNase P qPCR Normalization of viral DNA copies to the cell number