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1
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B19 IgG and IgM EIA Kit
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To test for B19 IgG and IgM antibodies in the serum samples.
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16299714
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2
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Nested PCR reagents
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To detect B19 DNA in the samples.
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3
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SSP Unitray HLA-ABC Kit
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For HLA class I tissue typing by multiplex PCR.
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4
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Overlapping peptides synthesized by Fmoc chemistry
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To represent the entire B19 NS1 and VP proteins for the IFN-g ELISpot assay.
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5
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Lithium-heparinized whole blood
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To isolate peripheral blood mononuclear cells (PBMCs).
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6
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Ficoll-Paque
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To separate PBMCs from whole blood.
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7
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Anti-IFNg monoclonal antibody (MAB 1-D1K, Mabtech)
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To coat the microtiter plates for IFN-g ELISpot assay.
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8
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Pooled human serum (BioWhittaker)
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To block microtiter plates.
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9
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RPMI-1640
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Medium for incubating PBMCs.
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10
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Fetal calf serum
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To supplement RPMI-1640 medium.
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11
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Streptomycin/penicillin
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To supplement RPMI-1640 medium.
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12
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L-glutamine
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To supplement RPMI-1640 medium.
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13
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Phytohemagglutinin (Sigma-Aldrich)
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Positive control in IFN-g ELISpot assay.
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14
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Biotin-linked anti-IFNg mAb (MAB 7-B6-1-Biotin, Mabtech)
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Secondary antibody in IFN-g ELISpot assay.
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15
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Streptavidin alkaline phosphatase (AP) (Bio-Rad)
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To conjugate with secondary antibody in IFN-g ELISpot assay.
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16
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AP conjugate substrate kit (Bio-Rad)
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To visualize the spots in the IFN-g ELISpot assay.
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