Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV020602 | GenBank Accession | DQ357064 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 4500 |
| Definition | Human parvovirus B19 isolate Vn147 NS1 (NS1), 7.5 kDa protein (NS1), VP1 (VP1), 9.5 kDa protein (VP1), and VP2 (VP2) genes, complete cds. | Submission Date | 2006-01-11 |
| Strain Name | Vn147 | Sampling Date | |
| Sampling Country | Vietnam | Location | Vietnam |
| Submitted By | Toan,N.L., Song,L.H., Duechting,A., Kandolf,R. and Bock,T.C. | Submitting Institution | Molecular Pathology, University Hospital Tuebingen, Liebermeistrasse 8, Tuebingen 72076, Germany |
| Original Host | human | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Primers (P5F, P5R, n-P5F, n-P5R) | Specifically amplify the subgenomic NS1/VP1u region of B19 for nested PCR and confirmatory PCR. | 16963753 | View Related Publication |
| 2 | DNA extraction kits | Extract DNA from serum samples. | ||
| 3 | PCR and nPCR reagents (mastermix, Taq polymerase, dNTPs) | To amplify B19 DNA sequences using PCR and nested PCR techniques. | ||
| 4 | WHO international standard for B19 DNA (NIBSC Code 99/800) | Standardize and determine the specificity and sensitivity of the B19 real-time qPCR assay. | ||
| 5 | B19-negative control plasmids | Serve as negative controls to ensure the accuracy and specificity of the qPCR assay. | ||
| 6 | BigDye Terminator cycle sequencing mix (Perkin Elmer) | Perform the sequencing reaction on purified PCR products. | ||
| 7 | Primer pairs for sequencing (Table 1 in the document) | Sequence specific regions of the B19 genome, including the NS1/VP1/VP2 region. |