Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV020268 GenBank Accession EU014200
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 251
Definition Human bocavirus isolate SIN07-NTU430 NS-1 gene, partial cds. Submission Date 2007-07-03
Strain Name SIN07-NTU430 Sampling Date from 2005-10 to 2007-03
Sampling Country Singapore Location the Kandang Kerbau Women's and Children's Hospital, Singapore
Submitted By Tan,B.H., Lim,E.A.S., Loo,L.H., Seah,S.G.K., Tee,N.W.S., Lin,R.T.P. and Sugrue,R.J. Submitting Institution Detection and Diagnostics Laboratory, Defence Medical and Environmental Research Institute, 27 Medical Drive, #13-00, Singapore 117510, Singapore
Original Host human Sample Type nasophar yngeal swabs
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Nasopharyngeal swabs Collected from pediatric patients admitted with acute respiratory infections. 19031441 View Related Publication
2 QIAamp viral RNA minikit Used for total nucleic acid extraction from the nasopharyngeal swabs.
3 QIAamp RNeasy minikit Used for total nucleic acid extraction from the nasopharyngeal swabs.
4 Platinum Taq DNA polymerase Used in PCR reaction mixture for detection of HBoV.
5 dNTPs Used in PCR reaction mixture for detection of HBoV.
6 MgCl2 Used in PCR reaction mixture for detection of HBoV.
7 SYBR Green RT-PCR reaction mix Used in PCR reaction mixture for detecting HCoV.
8 iScript reverse transcriptase Used in PCR reaction mixture for detecting HCoV.
9 Taqman probe Used in PCR reaction mixture for detection of RHV.
10 Superscript reverse transcriptase/Platinum Taq enzyme reaction mix Used in PCR reaction mixture for detection of RHV.
11 QIAquick gel extraction purification kit Used for purification of amplified PCR products from agarose gel.
12 BigDye Terminator cycle sequencing kit Used for DNA sequencing of the PCR products.
13 Ethidium bromide Used for visualizing PCR products by staining during gel analysis.