ParvoDB ID PV020055 GenBank Accession FM209185
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 318
Definition Human bocavirus human/1/2008/HUN partial VP1/VP2 gene for capsid protein, isolate human/1/2008/HUN. Submission Date 2008-08-30
Strain Name human/1/2008/HUN Sampling Date from 2007-10 to 2008-03
Sampling Country Hungary Location Hungary
Submitted By Takacs,M. Submitting Institution Takacs M., Division of Virology, National Center for Epidemiology, Gyali ut 2-6, H-1097, HUNGARY
Original Host Homo sapiens Sample Type Throat swabs and fecal samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Bartels Virus Respiratory Kit Direct immunofluorescence test for detection of respiratory pathogens (adenovirus, parainfluenzaviruses, respiratory syncytial virus, influenza A and B viruses, Chlamydia and Mycoplasma pneumoniae). 19349714 View Related Publication
2 Rota-Adeno Blister immunochromatography test (CerTest Biotec) Detection of gastroenteric pathogens, specifically rotavirus and adenovirus 40/41.
3 Ridascreen Norovirus Third Generation antigen detection ELISA kit (R-Biopharm) Detection of norovirus (calicivirus) in fecal samples.
4 Phenol/chloroform Deproteinization during DNA purification.
5 Proteinase K Digestion of proteins during DNA purification.
6 Isopropanol Precipitation of DNA during purification.
7 RNase- and DNase-free double distilled water (Gibco) Used as a solvent and for preparation of master mix in PCR.
8 ReadyMix (Sigma) Preparation of master mix for PCR.
9 PCR Clean up-M Kit (Viogene) Purification of PCR products for sequencing.
10 DYEnamic ET dye terminator kit (Pharmacia) Used for sequencing the PCR products.
11 BstAPI endonuclease Digestion of PCR products for restriction fragment length polymorphism (RFLP) analysis.