|
1
|
QIAamp DNA blood mini kit (Qiagen, Germany)
|
DNA extraction from patient samples.
|
https://www.researchgate.net/publication/273684108_Human_Bocavirus_Infection_in_Children_with_Acute_Respiratory_Infection_in_Nairobi_Kenya
|
View Related Publication
|
|
2
|
2% agarose gels
|
Electrophoresis to examine PCR amplicons.
|
|
3
|
Ethidium bromide
|
Staining of agarose gels to visualize PCR amplicons.
|
|
4
|
Virus transport medium (VTM)
|
Storage and transport of throat/oropharyngeal swabs.
|
|
5
|
QIAmp viral RNA kit (Qiagen, Valencia, CA, USA)
|
RNA extraction from patient samples.
|
|
6
|
10× PCR buffer (Life Technologies, Carlsbad, CA)
|
Component of the PCR reaction mix.
|
|
7
|
50 mM MgSO4 (Life Technologies, Carlsbad, CA)
|
Component of the PCR reaction mix.
|
|
8
|
2.5 mM dNTPs (Life Technologies, Carlsbad, CA)
|
Component of the PCR reaction mix.
|
|
9
|
AmpliTaq Gold DNA polymerase (Applied Biosystems)
|
Enzyme for the PCR reaction.
|
|
10
|
Primers (Sense 5’-TATGGCCAAGGCAATCGTCCAAG-3’ and antisense 5’-GCCGCGTGAACATGAGAAACAGA-3’)
|
Targeting a 254 bp region of the NS1 gene for HBoV detection.
|
|
11
|
Standard molecular markers
|
Size comparison in gel electrophoresis.
|
|
12
|
ExoSap (Amersham Biosciences, Cleveland, OH)
|
Purification of PCR products to remove unincorporated primers and dNTPs.
|
|
13
|
Big dye terminator (Applied Biosystems)
|
Sequencing of purified PCR products.
|
