Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV019713 GenBank Accession OK482576
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 5028
Definition Human erythrovirus V9 isolate BDN/18/181, complete genome. Submission Date 2021-10-08
Strain Name BDN/18/181 Sampling Date from 2016-01 to 2018-12
Sampling Country India Location state of West Bengal, India
Submitted By Sudharshan,S., Ghosh,A., Pal,A., Hameed,S.S., Desai,A.S. and Vasanthapuram,R. Submitting Institution Neurovirology, National Institute of Mental Health and Neurosciences, Hosur Road, Bangalore, Karnataka 560029, India
Original Host Homo sapiens Sample Type CSF of patients
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 ABsolute QPCR Mix (catalog no. AB-1133/A) For genotype-specific PCR in detecting Parvovirus B19 and its classification into genotypes. 35412386 View Related Publication
2 B19V primer F-CGCCTGGAACASTGAAACCC Forward primer for genotype-specific PCR detection of Parvovirus B19.
3 B19V primer R-TCAACCCCWACTAACAGTTC Reverse primer for genotype-specific PCR detection of Parvovirus B19.
4 Genotype 1 Probe Cy5-GTTGTAGCTGCATCGTGGGAAGA-BHQ2 Fluorescent probe for genotype-specific detection of Parvovirus B19 genotype 1.
5 Genotypes 2 and 3 Probe-ROX-GTGGTAGCCGCGTCGTGGGAGGA-BHQ2 Fluorescent probe for genotype-specific detection of Parvovirus B19 genotypes 2 and 3.
6 Multiplex PCR primers spanning entire B19V genome (designed using genotype 3 V9 NC_004295) Primers for amplifying the full genome or partial genome of Parvovirus B19 for sequencing.
7 Native barcoding kit (NBD104/NBD114, ONT) For barcoding amplicons during sequencing of PCR products.
8 Ligation kit (SQK-LSK-109, ONT) For ligation-based preparation of sequencing libraries.
9 FLO-MIN-106 flow cell Sequencing flow cell used on the MinION sequencer.