Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV019536 | GenBank Accession | FJ911592 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 447 |
| Definition | Human bocavirus isolate LZ614 nonfunctional NS1 (NS1) gene, partial sequence. | Submission Date | 2009-04-11 |
| Strain Name | LZ614 | Sampling Date | from 2007-12 to 2008-11 |
| Sampling Country | China | Location | Gansu Province, China, China |
| Submitted By | Song,J.R., Jin,Y., Xie,Z.P. and Gao,H.C. | Submitting Institution | Department of Viral Diarrhea, National Institute for Viral Disease Control and Prevention China CDC, 100 Ying-Xin St., Xuan-Wu District, Beijing 100052, China |
| Original Host | Homo sapiens | Sample Type | NPA samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp DNA mini kit (QIAGEN, Beijing, China) | DNA extraction from nasopharyngeal aspirates | 20113572 | View Related Publication |
| 2 | QIAamp viral RNA mini kit (QIAGEN, Beijing, China) | RNA extraction from nasopharyngeal aspirates | ||
| 3 | Random hexamer primers | Synthesis of cDNA from RNA samples | ||
| 4 | Primers 188F and 542R | PCR screening for human bocavirus (HBoV) | ||
| 5 | Primers sf1/sr1 and sf2/sr2 | Nested PCR for detecting HBoV2 | ||
| 6 | HBoV2 forward primer (SN1: 5′- ACCAGTGGGAGAACCACAAG-3′) | Amplification of a 563-bp fragment of the NP1 gene of HBoV2 | ||
| 7 | HBoV2 reverse primer (SN2: 5′-GGCATTTGTTTCCATGCTTT-3′) | Amplification of a 563-bp fragment of the NP1 gene of HBoV2 | ||
| 8 | Positive controls | To ensure accurate PCR results | ||
| 9 | Negative controls | To ensure accurate PCR results |