Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV019160 | GenBank Accession | HQ127219 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 289 |
| Definition | Human parvovirus B19 isolate RJ11 structural protein (VP1) gene, partial cds. | Submission Date | 2010-08-10 |
| Strain Name | RJ11 | Sampling Date | 2005 |
| Sampling Country | Brazil | Location | Brazil |
| Submitted By | Mendonca,M.C.L. and Couceiro,J.N.S.S. | Submitting Institution | Virologia, UFRJ, Avenida Carlos Chagas Filho 373, Rio de Janeiro, Rio de Janeiro 21941-902, Brazil |
| Original Host | Homo sapiens | Sample Type | serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | PCR primers (PVP1, PVP2, PVP3) | Amplifying a partial VP1-unique region of 327 bp in semi-nested PCR. | 21739041 | View Related Publication |
| 2 | Formamide buffer including 20 mM EDTA and 0.25% bromophenol blue dye | Used for mixing with PCR product in SSCP analysis. | ||
| 3 | 15% polyacrylamide gel in Tris-HCl buffer, pH 6.8 | Used for electrophoresis in SSCP analysis. | ||
| 4 | Silver staining | Visualizing samples after electrophoresis in SSCP and HMA analysis. | ||
| 5 | 15% polyacrylamide gel in Tris-HCl buffer, pH 8.8 | Used for electrophoresis in HMA analysis. | ||
| 6 | DNA prototypes (Au, T900, A6, Gh3051) | Used as standard samples for DNA genotypes 1, 2, and 3 respectively. | ||
| 7 | RNase-free water | Used for dissolving and preparing DNA samples. |