Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV019042 GenBank Accession L05209
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 170
Definition Parvovirus (donor P5) partial ORF. Submission Date 1993-06-12
Strain Name None Sampling Date
Sampling Country None Location None
Submitted By McOmish,F., Yap,P.L., Jordan,A., Hart,H., Cohen,B.J. and Simmonds,P. Submitting Institution J. Clin. Microbiol. 31 (2), 323-328 (1993)
Original Host Homo sapiens Sample Type serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Restriction endonucleases (EcoRI, BamHI, AvaI, BglII, Clal, EcoRV, HindIII, PstI, PvuI, PvuII, Sall, SstI, SstII, XbaI, and XhoI) Used for digesting DNA for mapping and sequencing 3701931 View Related Publication
2 T4 DNA ligase Used for ligating DNA fragments
3 T4 polynucleotide kinase Used for labeling DNA ends
4 DNA polymerase I (Klenow fragment) Used for DNA synthesis and filling-in reactions
5 Exonuclease III Used for generating nested deletions
6 Linkers Used for attaching specific sequences to DNA ends for cloning
7 Si nuclease Used for removing single-stranded tails
8 α-32P-deoxyribonucleoside triphosphates (dNTPs) Used for DNA sequencing
9 α-Thio-dNTPs (αS-dNTPs) Used for generating protected DNA ends in sequencing
10 NACS-52 columns Used for DNA purification
11 Alkaline lysis solutions Used for plasmid DNA isolation
12 CsCl density gradient Used for plasmid DNA purification
13 Phenol-CHCl3 Used for DNA extraction and purification
14 Ethanol Used for DNA precipitation
15 Synthetic deoxyribooligonucleotides (13- to 16-mers) Used as primers for DNA sequencing
1 Aliquots of plasma (200 μl) Collected and stored at -20°C prior to pooling and testing by PCR. 8432819 View Related Publication
2 Blood products from U.K. National Institute of Biological Standards and Controls (NIBSC) and Protein Fractionation Centre, Scottish National Blood Transfusion Service (SNBTS) Used to obtain freeze-dried preparations of factor VIII and factor IX and intravenous immunoglobulin (IgG).
3 Freeze-dried preparations of factor VIII and factor IX Stored at 4°C prior to reconstitution.
4 Intravenous immunoglobulin (IgG) Stored as a reconstituted aqueous solution (50 g/liter) at -20°C.
5 Lyophilized vials of factor VIII and factor IX Reconstituted as specified by the manufacturer immediately before use.
6 B19-positive plasma Used to spike intermediate-purity factor VIII 1:100 to investigate kinetics of B19 inactivation during heat treatment.
7 Primers PV1 to PV4 Used in the initial and nested PCR reactions to detect parvovirus B19 DNA.
8 Primers PV5 to PV8 Used for second reaction of the double PCR, amplifying part of the B19 genome encoding an antigenic region within the B19 nucleocapsid.
9 Water Used as the final solution for DNA extraction from plasma pools.
10 Ethidium bromide Used to stain PCR products for examination by agarose gel electrophoresis.
11 Agarose gel Used for electrophoresis to examine PCR products.
12 Positive and negative controls for PCR Ensured accuracy and validity of PCR results.