Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV018883 GenBank Accession OL519569
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 5419
Definition MAG: Human bocavirus 1 isolate xz003-boca-1, complete genome. Submission Date 2021-11-16
Strain Name xz003-boca-1 Sampling Date 2018
Sampling Country China Location Xuzhou, China
Submitted By Mao,Q. and Zhang,W. Submitting Institution School of Medicine, Jiangsu University, Xiangshan, Zhenjiang, Jiangsu 212013, China
Original Host Homo sapiens Sample Type swab samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Disposable swabs To collect pharyngeal swab samples. 36058285 View Related Publication
2 PBS (Phosphate Buffered Saline) To suspend the swab samples after collection.
3 0.45-μm filter (Millipore) To filter the supernatant to remove eukaryotic and bacterial cell size particles.
4 Turbo DNase (Ambion) To remove unprotected nucleic acids.
5 Baseline-ZERO DNase (Epicentre) To remove unprotected nucleic acids.
6 Benzonase (Novagen) To remove unprotected nucleic acids.
7 RNase (Fermentas) To remove unprotected nucleic acids.
8 QIAamp Viral RNA Mini Kit (QIAGEN) To extract total nucleic acids (DNA and RNA) from the filtered samples.
9 Super-Script III (Invitrogen) For reverse transcription of RNA into the first strand of cDNA.
10 Random hexamer primer To amplify nucleic acids during reverse transcription.
11 Large (Klenow) fragment (NEB) For second strand cDNA synthesis and conversion of ssDNA to dsDNA.
12 Nextera XT DNA Sample Preparation Kit (Illumina) For constructing libraries for sequencing.
13 Primer sets (Supplementary Table 1) For nested primer PCR to bridge gaps between contigs.
14 rTaq (TaKaRa) For the PCR enzyme used in reactions.