Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV018626 GenBank Accession LR721652
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 748
Definition Human bocavirus 1 partial NP1, VP1/VP2 gene for Structural and Non-structural proteins, segment NP1, VP1/VP2, isolate KW-HBo-V 65. Submission Date 2019-09-19
Strain Name KW-HBo-V 65 Sampling Date 2019-03-21
Sampling Country Kuwait Location Kuwait
Submitted By Madi,M.N. Submitting Institution Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait
Original Host Homo sapiens Sample Type respiratory samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Nasopharyngeal aspirates/wash, tracheal aspirates and throat swabs Collected from patients for respiratory sample analysis. 32579103 View Related Publication
2 Roche MagNA Pure LC system Used to extract viral RNA or DNA from respiratory samples.
3 Fast Track kit (Fast Track Diagnostics, Luxembourg) Used for one-step multiplex real-time PCR assay to detect various respiratory viruses including HBoV.
4 Primers HBoV-NP1/VP/1 and HBoV-NP1/VP/R Used to screen HBoV-positive samples for the NP1, VP1, and VP2 gene boundary.
5 Agarose gel (1.5% W/V) Used for electrophoresis analysis of the PCR amplicon.
6 QIAquick gel extraction kit (Qiagen) Used to purify PCR products.
7 ABI 3500/3500xL genetic analyzer (PE Applied Biosystems, Inc.) Used for sequencing purified products in forward and reverse directions.
8 ABI Prism BigDye Terminator Cycle Sequencing Ready Reaction kit (PE Applied Biosystems, Inc.) Used for performing sequencing reactions.