Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV018611 GenBank Accession DQ200648
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 266
Definition Human bocavirus strain QPID04-0007 NS1 gene, partial cds. Submission Date 2005-09-10
Strain Name QPID04-0007 Sampling Date from 2004-05 to 2004-08
Sampling Country Australia Location Queensland, Australia
Submitted By Mackay,I.M. Submitting Institution QPID Laboratories, Sir Albert Sakzewski Virus Research Centre, c/o Royal Children's Hospital, Herston Rd, Brisbane, Qld 4029, Australia
Original Host Homo sapiens Sample Type nasopharyngeal aspirates
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Nasopharyngeal aspirates (NPA) Specimens collected from ill patients for testing. 16257260 View Related Publication
2 Bronchoalveolar lavage Specimens collected from ill patients for testing.
3 Bronchial washings Specimens collected from ill patients for testing.
4 Culture-amplified direct fluorescent assay Diagnostic assay to detect common microbial respiratory pathogens.
5 RT-PCR Diagnostic assay to detect common microbial respiratory pathogens such as HRSV, human adenoviruses, HPIV-1, 2 and 3 and influenza viruses A and B.
6 Selective culture media To isolate bacterial pathogens including Pseudomonas aeruginosa, Streptococcus species, Haemophilus influenzae, Neisseria species, Staphylococcus species, Legionella pneumophila, and Candida albicans.
7 Single-tube RT-PCR and PCR amplifications (OneStep RT-PCR and HotStarTaq kits, QIAGEN) To detect and amplify viral genetic material from specimens.
8 Primers To target specific genes in the pan-HCoV, HCoV-HKU1, HCoV-OC43, and HBoV assays.
9 QIAquick (QIAGEN, Australia) To purify the amplicon directly from the reaction mixture.
10 ABI PRISM BigDye cycle sequencing kit (Perkin Elmer Applied Biosystems Division, USA) To perform nucleotide sequencing reactions on both strands of the amplicon.
11 Applied Biosystems 3130 ×l capillary electrophoresis (CE) genetic analyser To determine nucleotide sequences.