Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV018597 | GenBank Accession | DQ296624 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 311 |
| Definition | Human bocavirus isolate JPBS03-219 NP-1 gene, partial cds. | Submission Date | 2005-11-15 |
| Strain Name | JPBS03-219 | Sampling Date | |
| Sampling Country | Japan | Location | Sapporo, Japan |
| Submitted By | Ma,X., Endo,R., Ishiguro,N., Ebihara,T., Ishiko,H., Ariga,T. and Kikuta,H. | Submitting Institution | Department of Pediatrics, Hokkaido University Graduate School of Medicine, N-15, W-7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan |
| Original Host | Homo sapiens | Sample Type | nasopharyngeal swab samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Nasopharyngeal swab samples | Collected from children with lower respiratory tract infections for investigation. | 16517912 | View Related Publication |
| 2 | Chomczynski’s protocol | Used for the extraction of RNA and DNA from each sample. | ||
| 3 | PCR primers (forward primer: 5'-GAGCTCTGTAAGTACTATTAC-3', reverse primer: 5'-CTCTGTGTTGACTGAATACAG-3') | Used for the detection and sequencing of HBoV. | ||
| 4 | PCR mixture components: 100 μmol each deoxyribonucleotide, 1.0 U AmpliTaq Gold, 50 mmol potassium chloride/liter, 10 mmol Tris-HCl (pH 8.3)/liter, 1.5 mmol magnesium chloride/liter, 0.01% (wt/vol) gelatin, 10 pmol each primer | Used for performing PCR to detect HBoV DNA. | ||
| 5 | BigDye terminator cycle sequencing ready reaction kit | Used for direct sequencing of PCR products. | ||
| 6 | ABI Prism 310 genetic analyzer | Used for analyzing the DNA sequences of the PCR products. | ||
| 7 | LLC-MK2 cells | Used for virus culture to attempt HBoV isolation from nasopharyngeal samples, although found inappropriate for initial isolation. |