Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV018501 GenBank Accession MT534176
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 286
Definition Erythroparvovirus primate1 isolate HEM194 VP1/VP2 protein gene, partial cds. Submission Date 2020-05-28
Strain Name HEM194 Sampling Date 2015
Sampling Country Brazil Location Brazil
Submitted By Alves,A.D.R., Amado,L.A. and Pinto,M.A. Submitting Institution Virology, Oswaldo Cruz Institute, Brasil Avenue, 4365 - Manguinhos, Rio de Janeiro, Rio de Janeiro, Rio de Janeiro 21040900, Brasil
Original Host Homo sapiens Sample Type serum samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) DNA extraction from serum samples 32927082 View Related Publication
2 TaqMan system (Applied Biosystems 7500 Real-Time PCR System, Applied Biosystems, USA) Real-time PCR for molecular detection and quantification of B19V
3 Synthetic standard curve of the B19V NS1 region (IDT, USA) Absolute quantification in real-time PCR
4 Primers for the NS1 region (nt 1905F and 1987R) and a single labelled 50 FAM probe (nt 1925–1948, GenBank: NC_000883.2) Real-time PCR amplification
5 ELISA kits (VirionSerion, Germany) Detection of anti-B19V IgM and IgG antibodies
6 Primer pairs VP1/VP1R (nt 3502–3883, GenBank: NC_000883.2) and VP2/VP2R (nt 3541–3867, GenBank: NC_000883.2) Nested PCR for B19V genotyping
7 QIAquick gel extraction (Qiagen, Germany) Purification of nested PCR products
8 BigDye terminator v. 1.1 cycle sequencing kit (Applied Biosystems, USA) Sequencing of nested PCR products
9 HBsAg, anti-HBc, anti-HBs, and anti-HCV ELISA kits (Euroimmun, Germany) Testing for hepatitis B and C markers