Panoramic Parvovirus Strain Discovery Data Chain

Download Strain's Sequence
Feedback for This Record
ParvoDB ID PV018499 GenBank Accession MG572795
Subfamily Parvovirinae Genus Erythroparvovirus
Species Erythroparvovirus primate1 Sequence Length 423
Definition Erythroparvovirus primate1 isolate FHA011 VP2 gene, partial cds. Submission Date 2017-11-24
Strain Name FHA011 Sampling Date from 2004 to 2009
Sampling Country Brazil Location Brazil
Submitted By Alves,A.D.R., Amado Leon,L.A. and Pinto,M.A. Submitting Institution Virology, Oswaldo Cruz Institute, Brasil Avenue, 4365 - Manguinhos, Rio de Janeiro 21040900, Brasil
Original Host Homo sapiens Sample Type Serum and liver samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Anti-HAV IgM ELISA (Abbott Laboratories, IL, USA) Screening for hepatitis A virus 32286103 View Related Publication
2 Vikia HBsAg ELISA (bioMérieux, Craponne, France) Screening for hepatitis B surface antigen
3 Murex anti-HCV version 4.0 ELISA (Diasorin, Midrand, South Africa) Screening for hepatitis C virus
4 bioELISA HEV IgM version 3.0 ELISA (Biokit, Barcelona, Spain) Screening for hepatitis E virus
5 Rapid tests (DPP, Bio-Manguinhos, Rio de Janeiro, Brazil; SD Bioline, Standard Diagnostics, Suwon, South Korea) Screening for syphilis, HIV-1/2, dengue, and leptospirosis infections
6 Chemiluminescent ELISA (Abcam, MA, USA) Screening for CMV, Epstein-Barr virus, and antinuclear antibodies (ANAs)
7 Multiplex qPCR TaqMan assay Investigation of human herpes virus 1 and 2 (HHV-1 and HHV-2) and human herpes virus 6
8 Commercial colorimetric assay kits (Abbott) Biochemical analysis to determine the levels of liver enzymes and total bilirubin
9 Anti-B19V IgG and IgM ELISA (Biotrin International Ltd., Dublin, Ireland) Evaluation of anti-B19V IgG and IgM antibodies
10 QIAamp DNA Blood Mini kit (Qiagen, Hilden, Germany) Extraction of B19V DNA from sera
11 QIAamp Fast DNA Tissue kit (Qiagen, Hilden, Germany) Extraction of B19V DNA from liver explants
12 7500 Real-Time PCR system (Applied Biosystems, MA, USA) Quantification of B19V load by real-time PCR using TaqMan system
13 BigDye Terminator v. 1.1 cycle sequencing kit (Applied Biosystems) Direct sequencing of PCR products
14 QIAquick gel extraction kit (Qiagen) Purification of 476-bp PCR products
15 RNA extraction using Ambion MicroPoly(A)Purist (Thermo Fisher Scientific, MA, USA) Selection of mRNA from total RNA preparations
16 DNase I digestion (final concentration, 90 U/ml; Promega Co., WI, USA) Digestion of residual DNA before cDNA synthesis
17 SuperScript III Reverse Transcriptase (RT) (Life Technologies Corp., CA, USA) Synthesis of cDNA from purified mRNA for RT-PCR
18 Sybr Green qRT-PCR Quantification of B19V NS1 transcript expression