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1
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0.45-μm filter (Merck Millipore Ltd., Cork, Ireland)
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Filtered the clarified samples to remove larger debris for viral metagenomics.
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31614994
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2
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DNAse and RNAse enzymes
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Digested free nucleic acids to enrich for viral nucleic acids protected within viral capsids.
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3
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MagMAX Viral RNA Isolation Kit (Ambion, Inc, Austin, TX, USA)
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Nucleic acid extraction.
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4
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Nextera XT Sample Preparation Kit (Illumina)
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Generated a library for Illumina MiSeq sequencing.
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5
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Roche 480 thermocycler
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Used for real-time PCR amplification.
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6
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QuantiFast Probe PCR Master Mix (Qiagen, Hilden, Germany)
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Reaction mix in real-time PCR.
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7
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Primers and probes
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Specific detection and quantification of viral nucleic acids in samples by real-time PCR.
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8
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Oligonucleotides representing the PCR target regions
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Determined the sensitivity of real-time PCR assays.
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9
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Geneious R10 program
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Alignment of reads and contigs to reference viral genomes for sequence analysis. Used here contextually but essentially part of kit/protocol.
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