Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017988 | GenBank Accession | EF560212 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 279 |
| Definition | Human bocavirus strain MC-8 NS-1 gene, partial cds. | Submission Date | 2007-04-16 |
| Strain Name | MC-8 | Sampling Date | from 2003-01 to 2005-12 |
| Sampling Country | Brazil | Location | Brazil |
| Submitted By | Albuquerque,M.C.M. and Santos,N. | Submitting Institution | Departamento de Virologia, Instituto de Microbiologia - UFRJ, CCS, Bl I, Cidade Universitaria, I Fundao, Rio de Janeiro, RJ 21941-590, Brazil |
| Original Host | None | Sample Type | fecal specimens |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Phosphate-buffered saline (pH 7.2) | Preparation of 10% (w/v) stool suspensions. | 18217564 | View Related Publication |
| 2 | Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) | DNA extraction from stool suspensions. | ||
| 3 | HBoV 01.2 forward primer (5′-TATGGCCAAGGCAATCGTCCAAG-3′) | PCR amplification for the nonstructural protein 1 gene of HBoV. | ||
| 4 | HBoV 02.2 reverse primer (5′-GCCGCGTGAACATGAGAAACAGA-3′) | PCR amplification for the nonstructural protein 1 gene of HBoV. | ||
| 5 | Ethidium bromide | Staining for agarose gel electrophoresis to detect PCR products. | ||
| 6 | Wizard SV gel and PCR Clean-Up system kit (Promega) | Purification of amplified DNAs of PCR-positive samples. | ||
| 7 | BigDye Terminator Cycle Sequencing Kit | Sequencing of purified PCR products. |