Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017931 | GenBank Accession | NC_024888 |
| Subfamily | Parvovirinae | Genus | Protoparvovirus |
| Species | Protoparvovirus primate1 | Sequence Length | 4733 |
| Definition | Bufavirus-3 genes for NS1, putative VP1, hypothetical protein, VP2, complete cds, strain: BTN-63. | Submission Date | 2013-08-23 |
| Strain Name | BTN-63 | Sampling Date | 2010-04-26 |
| Sampling Country | Bhutan | Location | Bhutan |
| Submitted By | Ahmed,K., Yahiro,T. and Matsumoto,T. | Submitting Institution | Contact:Takaaki Yahiro Faculty of Medicine, Oita University, Microbiology; 1-1 Idaigaoka, Hasama, Yufu, Oita 879-5593, Japan |
| Original Host | Homo sapiens | Sample Type | fecal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp Viral RNA Mini Kit (QIAGEN Sciences, Valencia, CA, USA) | Extraction of viral genomic DNA from fecal samples. | 24856373 | View Related Publication |
| 2 | Primers for nested PCR targeting the NS1 region | Determination of presence of BuV. | ||
| 3 | Primers constructed from consensus regions of BuV1 whole-genome sequences | Whole-genome sequencing of BuV3. | ||
| 4 | Primers for PCR testing | Testing for norovirus, bocavirus, adenovirus, astrovirus, salivirus, cosavirus, and aichivirus. | ||
| 5 | Enzyme immunoassay | Testing for rotavirus. | ||
| 6 | BigDye Terminator v3.1 Cycle Sequencing kit (Applied Biosystems, Foster City, CA USA) | Nucleotide sequencing. |