Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017894 | GenBank Accession | AB761128 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate2 | Sequence Length | 454 |
| Definition | Human bocavirus 2A VP1 gene for capsid protein, partial cds, strain: ICMH623HBoV. | Submission Date | 2012-11-05 |
| Strain Name | ICMH623HBoV | Sampling Date | from 2005-07 to 2006-06 |
| Sampling Country | Bangladesh | Location | Child and Mother Health, Matuail, Dhaka, Bangladesh |
| Submitted By | Ahmed,K. and Mitui,M.T. | Submitting Institution | Contact:Kamruddin Ahmed Oita University, Research Promotion Institute; 1-1 Hasama machi, Yufu, Oita 879-5593, Japan Fax :81-97-586-5719 |
| Original Host | Homo sapiens, child under 5 years old | Sample Type | faecal samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) | Used for nucleic acid extraction from 10% fecal suspension | 24105741 | View Related Publication |
| 2 | TE buffer | Used for eluting nucleic acids post-extraction | ||
| 3 | -30°C storage | Storage condition for eluted nucleic acids | ||
| 4 | PCR Master Mix (Promega Corporation, Madison, WI) | Used for conventional polymerase chain reaction (PCR) | ||
| 5 | AccessQuick RT-PCR System (Promega Corporation, Madison, WI) | Used for reverse transcription (RT)-PCR | ||
| 6 | Negative control containing nuclease-free water | Used to determine the presence of contaminants during PCR | ||
| 7 | BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA) | Used for nucleotide sequencing of purified amplicons | ||
| 8 | ABI Prism 3130 Genetic Analyzer (Applied Biosystems) | Automated genetic analyzer used to run sequencing products |