Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017676 | GenBank Accession | AB126271 |
| Subfamily | Parvovirinae | Genus | Erythroparvovirus |
| Species | Erythroparvovirus primate1 | Sequence Length | 4466 |
| Definition | Human parvovirus B19 NS1, VP1, VP2 genes for NS1 protein, VP1 protein, VP2 protein, complete cds, isolate: B19-AN87. | Submission Date | 2003-11-17 |
| Strain Name | B19-AN87 | Sampling Date | from 1994 to 2000 |
| Sampling Country | Japan | Location | Kyoto University Hospital, Japan |
| Submitted By | Abe,K., Aiba,N., Kiuchi,T. and Tanaka,K. | Submitting Institution | Contact:Kenji Abe National Institute of Infectious Diseases, Department of Pathology; Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan URL :http://www.nih.go.jp |
| Original Host | None | Sample Type | liver tissues and serum samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | SepaGene RV-R Kit (Sanko-Junyaku, Tokyo, Japan) | Extraction of nucleic acids (DNA/RNA) from frozen liver tissues and serum. | 17479159 | View Related Publication |
| 2 | RNase-free water | Resuspending the resulting pellet after nucleic acid extraction. | ||
| 3 | Primers PV1, PV1R, PV2, and PV2R | Used for nested PCR to screen B19 DNA. | ||
| 4 | AmpliTaq Gold (Perkin Elmer, Norwalk, Conn.) | PCR amplification. | ||
| 5 | Blend-Taq-Plus DNA polymerase (TOYOBO Co., Tokyo, Japan) | PCR amplification. | ||
| 6 | QIAquick gel extraction kit (Qiagen Inc., Chatsworth, Calif.) | Purification of amplicons obtained from PCR. | ||
| 7 | ABI PRISMTM Big Dye Terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer) | Direct sequencing of recovered amplicons. | ||
| 8 | RQ I DNase (Promega Co., Madison, Wis.) | Digestion of RNA to remove any DNA before reverse transcription. | ||
| 9 | Moloney murine leukemia virus reverse transcriptase (Promega Co.) | Synthesis of cDNA from pretreated RNA for detection of B19 mRNA. |