Panoramic Parvovirus Strain Discovery Data Chain
| ParvoDB ID | PV017647 | GenBank Accession | MF977921 |
| Subfamily | Parvovirinae | Genus | Bocaparvovirus |
| Species | Bocaparvovirus primate1 | Sequence Length | 378 |
| Definition | Human bocavirus isolate #Taif-8 coat protein VP1 gene, partial cds. | Submission Date | 2017-09-19 |
| Strain Name | #Taif-8 | Sampling Date | 2016-07-11 |
| Sampling Country | Saudi Arabia | Location | King Faisal Hospital, Al-Taif, Saudi Arabia |
| Submitted By | Abdel-Moneim,A.S., Mahfouz,M.E. and Zytouni,D.M. | Submitting Institution | Microbiology Department, College of Medicine, Taif University, El-Setteen Street, Al-Haweyia, Al-Taif, Makkah 21944, Saudi Arabia |
| Original Host | Homo sapiens | Sample Type | blood samples |
| Standardized Host Name | Homo sapiens | Host Tax Rank | species |
| Standardized Host Class | Mammalia | Standardized Host Order | Primates |
| Environmental Origin | - N/A - | Reagent Origin | - N/A - |
| Number | Reagent/Consumables | Purpose | PMID/Url | Reference |
|---|---|---|---|---|
| 1 | Bio-Rad ELISA kits | Used for screening blood donors for common bloodborne pathogens (HBV, HCV, HIV, HTLV). | 29489915 | View Related Publication |
| 2 | DNA extraction kit (Thermo Scientific, Wilmington, DE) | Used for extracting genomic DNA from whole blood samples. | ||
| 3 | PCR mastermix (Solis BioDyne, Inc., Estonia) | Used for conducting polymerase chain reaction (PCR) amplification. | ||
| 4 | Primers (VP/NC For: 5’-AGCTGTGAGATTGTATGGGAAG-3’, PanBoca Rev: 5’-AAAACAGCTCCCCCCACAAT-3’) | Used for amplifying a highly conserved sequence in the VP1 gene and the non-coding region of HBoV. | ||
| 5 | Agarose gels (2%) | Used for electrophoresis to analyze PCR amplicons. | ||
| 6 | HBoV real-time kit (Life River, Shanghai, China) | Used for quantitatively screening HBoV positive samples by real-time PCR. | ||
| 7 | Wizard SV Gel extraction kit (Promega, UK) | Used for extracting DNA from the gel bands of PCR amplicons. |