Panoramic Parvovirus Strain Discovery Data Chain

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ParvoDB ID PV017636 GenBank Accession KM577336
Subfamily Parvovirinae Genus Bocaparvovirus
Species Bocaparvovirus primate1 Sequence Length 383
Definition Human bocavirus 1 strain Eg/BSU-4 coat protein VP1 (VP1) gene, partial cds. Submission Date 2014-09-08
Strain Name Eg/BSU-4 Sampling Date 2014-01-15
Sampling Country Egypt Location CairoGovernorate, Egypt
Submitted By Abdel-Moneim,A.S., Kamel,M.M., Hamed,D.H., Hassan,S.S., Soliman,M.S., Al-Quraishy,S.A. and El Kholy,A.A. Submitting Institution Virology Department, Faculty of Veterinary Medicine, Beni-Suef University, Mokbel, Beni-Suef 62511, Egypt
Original Host Homo sapiens Sample Type Nasopharyngeal swab samples
Standardized Host Name Homo sapiens Host Tax Rank species
Standardized Host Class Mammalia Standardized Host Order Primates
Environmental Origin - N/A - Reagent Origin - N/A -
Number Reagent/Consumables Purpose PMID/Url Reference
1 Universal virus transport medium (UTMTM, COPAN, USA) To store individual nasopharyngeal swab samples. 26658621 View Related Publication
2 Viral DNA/RNA extraction kit (Koma Bioteck Inc., Seoul, Korea) To extract viral nucleic acid from nasopharyngeal swab samples.
3 Commercial HBoV real-time PCR kit (Life River, Shanghai, China) To perform real-time PCR for the detection of Human bocavirus.
4 NP1 primer set Used in conventional PCR for amplification of NP1 gene of HBoV.
5 VP1/2 primer set Used in conventional PCR for amplification of VP1/2 gene of HBoV.
6 Newly developed VP/NC primer set Used in conventional PCR for amplification of VP/NC gene of HBoV, designed in this study.
7 PCR master mix (Solis BioDyne, Inc., Estonia) To perform PCR amplification with the designated primer pairs.
8 Positive and negative controls provided in the kits To ensure the reliability and accuracy of the PCR results.
9 Gel extraction kit (Koma Bioteck, Inc., Seoul, Korea) To purify positive PCR amplicons.